Search Keywords: GST Pichia pastoris Search date: 1/23/06

Interesting Abstracts:

• Biotechnol Lett. 2004 Mar;26(5):443-9. Expression of GST-fused kinase domain of human Csk homologous kinase from Pichia pastoris facilitates easy purification.

Murthy TV.

Division of Experimental Medicine, Harvard Institutes of Medicine, 4-Blackfan Circle, Boston, MA 02115, USA. tmurthy@hms.harvard.edu

Human Csk Homologous Kinase (CHK), a protein of 527 amino acid residues, is involved in suppression of breast tumors. The kinase domain of CHK (amino acid residues 228 to 485) expressed with C-terminal 6HIS fusion in Pichia pastoris is heavily glycosylated. Expression of the C-terminal 6HIS fused kinase domain of CHK, with an N-terminal glutathione S-transferase fusion, in Pichia pastoris alleviated the hyperglycosylation. The expressed protein was purified by affinity chromatography to 1 mg l(-1) culture and remained active. A simple plate assay to identify colonies of P. pastoris expressing the recombinant protein is also presented.

PMID: 15104145 [PubMed - indexed for MEDLINE]

-- DaniPershouse - 23 Jan 2007